Alex Young has published his second article from his MSc. While working to adapt flourescent in situ hybridization (FISH) methods to work with Lymnaea stagnalis, two things became apparent. There’s a fair number of published protocols out there, and no one has consolidated all the variants in one location to help anyone in our position. So, he decided to do it himself – adding in the expertise of Dan Jackson, we’ve put together exactly what the title says it is: a technical review and guide to RNA FISH. Hopefully others looking to work with FISH for the first time or adapt an established protocol will find this helpful.
Young, A.P., Jackson, D.J., and Wyeth, R.C. 2020. A technical review and guide to RNA fluorescence in situ hybridization. PeerJ 8: e8806. PeerJ Inc. doi:10.7717/peerj.8806.