Author: Russell Wyeth

Biofouling on graphene-based coatings

Post #3 Part 2 in a series from students working in the Wyeth lab this summer. This time it’s Mike Murtaugh working with Tia Landry and Aaron Cogger – working on several biofouling projects.

More from Mike…

In the biofouling research group we are constantly testing new antifouling coatings, checking several aspects, such as toxicity, the ease with which fouling is released and in situ overall effectiveness. A new batch of state-of-the-art coatings has recently been deployed at Port Hawkesbury to be analyzed weekly and compared to control plates. We are excited to see how much time can go by before these plates start accumulating visible biomass and what species will be the first to colonize, if any. Interesting results have been obtained from a coating tested during spring, which was made with one goal in mind, to prevent barnacles from settling. This task has been proven easier said than done in the past, however this novel coating showed very promising results, as seen in the image comparing a control to the coating. Can you count how many barnacles are attached to the control plate? How about on the coating?

Fig. 3. Five-week-old experimental plates deployed during spring at Arisaig harbor. There is noticeable recruitment of barnacle on the control plate (1), while no barnacles were recorded to settle on the coated plate (2). 

There is no one way to prevent biofouling, just as there is no one way it is formed. Because of this, its control will most likely come from a combination of various methods or specific treatments that will vary between locations and fouling communities. There is still much to be learned and here in the Wyeth Lab we are anxious to learn as much as we can about this challenging subject, to help find a solution to this age-old problem. 

Biofouling community recruitment and UV light

Post #3 Part 1 in a series from students working in the Wyeth lab this summer. This time it’s Mike Murtaugh working with Tia Landry and Aaron Cogger – working on several biofouling projects.

Mike says: as part of the biofouling research team in the Wyeth Lab, my master’s degree project focusses on contributing to the body of knowledge regarding the use of ultraviolet (UV) light for antifouling purposes and it has provided me with a fascinating view into the life history of the many biofouling members. By recording early recruitment and larval availability throughout the summer months of last year, many larval stages with intriguing shapes have been photographed and paired with their benthic counterparts.

This image exhibits members of the meroplanktonic community, along with three early recruits. Can you tell what larval type turns into each recruit? 

Meroplankton are images 1-8 and early biofouling members are A-C – all are from Nova Scotian coastal waters (scale bars = 250 µm). The eight members of the meroplanktonic community are: (1) Echinopluteus larva; (2) Ophiopluteus larva; (3) Auricularia larva; (4) Bipinnaria larva; (5) Cyphonautes larva; (6) Anenome larva; (7) Pilidium larva; and (8) Cyprid larva; and the three members of a one-week-old biofouling community are: (A) Ophiothrix fragilis (matches with #2); (B) Semibalanus balanoides (matches with #8); and (C) Membranipora membranacea (matches #5) 

Additionally, I am assessing the effect that a minimal UV light treatment has on biofouling communities, by tracking biofouling development on glass surfaces with and without a UV treatment at two Nova Scotian locations through photo-analysis. With these methods, I have found some interesting results from last summer, including changes in community composition caused by the UV treatment which subsequently affected biofouling diversity. Although these results are quite interesting and have important implications, this summer I will repeat my methodology, expecting to expand on these results and further test my hypotheses.

Research Technician Position Available

The Centre for Biofouling Research (CBR) at St. Francis Xavier University is seeking one or two research technicians. Our fundamental and applied research program explores both marine biofouling (the growth of unwanted organisms on marine infrastructure) and novel low-toxicity approaches to antifouling (the mitigation of biofouling). The technician(s) will help coordinate and participate in several ongoing research projects with respect to biofouling and environmentally-friendly antifouling. The position(s) will be primarily focused on supporting our research projects in collaboration with GIT Coatings, who are developing graphene-based coatings. Projects will include both field trials of newly developed coatings and lab-based toxicity assays. The technician will work as part of the biofouling team of students, all working with the supervision of Dr. Russell Wyeth. Activities working towards completion of the project will entail working as a group to plan and implement field and lab work, undertake sampling, and, once completed, prepare oral and written communications about the project(s). Administrative tasks in support of the supervisor will also be involved, as well as opportunities to engage with other active research projects.

Duties and responsibilities

  • help the project lead with overall organization of research activities
  • help with specific project planning and administration
  • team work with other student researchers
  • data analysis
  • materials and equipment management
  • help with report and manuscript writing
  • communicate with other stakeholders in the fields of biofouling and antifouling

Essential knowledge and skills

  • ability to work independently
  • meticulous organization
  • time management
  • effective oral and written communication

Desirable knowledge and skills:

  • principles of marine biofouling
  • antifouling technologies
  • research experience
  • reading and summarizing scientific literature
  • digital photography
  • toxicity tests
  • brine shrimp culture
  • image processing
  • digital video production
  • software: MS Excel, SPSS, R
  • statistical analysis
  • scientific presentation
  • scientific writing
  • marine field work experience

Education and/or Certification required

  • Bachelor of Science
  • Workplace Hazardous Materials Information System (WHMIS) or Globally Harmonized System of Classification and Labelling of Chemicals (GHS)

Position Details and Application Procedures

  • Either one or two positions available, depending on funding
  • Eligibility: applicants must be completing a science degree or have graduate from a science degreee within the last 18 months.
  • Start date: Aug 6, 2024 or later (some flexibility)
  • Salary: $17/hour
  • Duration: 4 months minimum, extendable upon review for up to at least 12 months
  • Remote work possible, but at least some in-person work preferred
  • Deadline: review of applications will begin July 17, and continue until position(s) filled

To apply, please send a transcript, resume, and cover letter to Russell Wyeth – rwyeth@stfx.ca

The cover letter should summarize:

  • your interest in the position
  • relevance of your experience
  • the graduation date and discipline of your most recent science degree
  • your citizenship/immigration status in Canada

Juvenile Lobster Behaviour

Post #2 in a series from students working in the Wyeth lab this summer. Next up are Ryan Bergman, James Hanlon and Aidan McGowan, who are surveying juvenile lobster behaviour in the near shore environments around the southern Gulf of St. Lawerence

Our research focuses on s juvenile lobster abundance and behavior along the Scallop Buffer Zone. These protected zones along Nova Scotia’s northern coastline encompass pristine habitat for juvenile lobsters, which tend to settle in complex rocky habitats. Using underwater videography, we aim to explore the distribution of juvenile lobsters along the Scallop Buffer Zone. Additionally, we aim to use these videos to learn about the behavioral patterns of juvenile lobsters in the presence of bait. 

After selecting a beach to survey for lobsters, we head into the field and setup our cameras. To position them in the water, the cameras are placed on tripods made from metal poles roughly 2 meters tall. We then carry them into the water, attach buoys to the head and leg poles, and swim the tripod out as it floats in the water. In wetsuits and snorkeling gear, we search for ideal filming locations that lobsters may settle – for example, near the edges of seaweed and over rock piles. 

Filming over four hours of footage each day, we analyze our video data by first counting and measuring the size of lobsters. This allows us to identify habitats that juvenile lobsters frequently use and find beach sites that provide such favorable conditions. Using this contextual data, we will then document the behaviors of the juveniles as they are drawn out of their shelters with bait. This will us to classify how juvenile’s behaviour varies across different habitats and population densities. 

Victoria Tweedie-Pitre

My name is Victoria Tweedie-Pitre, and I am a recent graduate from StFX with a Joint Honours in Biology and Chemistry. I am so excited to be working for a second summer in the Wyeth Lab where I focuses on exploring the intricate neuroanatomy of the great pond snail, Lymnaea stagnalis. Using cutting-edge techniques such as in situ Hybridization Chain Reaction, I categorize and map different neuron types based on their association with neurotransmitter synthesis. I have always had a passion for exploration and love for marine biology, and I am excited to continue channeling this enthusiasm into my research. After completing my work here, I am returning home to the Northwest Territories to engage in a low arctic terrestrial ecology project before pursuing a master’s degree. I am incredibly grateful to be a part of the Wyeth Lab, and I am eager to continue developing skills that will be invaluable throughout my career and life.

Hermissenda field behaviour

Post #1 in a series of posts from students working in the Wyeth lab this summer. First up is Lexy Reinhardt, who is collecting data for her MSc thesis focused on the navigation behaviour of the sea slug Hermissenda crassicornis. Take it away Lexy…

Close-up of Hermissenda crassicornis, the long-horned nudibranch.
Example of field video data capturing Hermissenda behaviour

Dr. Wyeth and I are currently at the Bamfield Marine Science Centre collecting underwater videos of sea slugs in their natural habitat for my MSc thesis. My ongoing research project focuses on the behavior and navigation of Hermissenda crassicornis, commonly known as the thick-horned nudibranch. 

For the past three weeks, we have undertaken daily SCUBA dives to deploy and recover underwater video cameras mounted on tripods. These cameras are strategically placed to capture video of the nudibranchs as they move through their natural environment.

The high-quality video, the likes of which have never been captured before, will enable us to conduct detailed analyses of movement patters and also contribute to the broader understanding of Hermissenda crassicornis behaviour. Though we are still in the early stages of data collection, we’ve already begun to observe some intriguing patterns and behaviours! 

In the coming weeks, we will continue our daily dives and video recordings. Our goal is to gather a comprehensive set of videos that will allow us to identify specific navigational trends and behavioural characteristics associated with different flow environments and chemical stimulus sources. 

We are thrilled with our progress so far and look forward to sharing more detailed findings as our project advances. Stay tuned for further updates!

Yulia Reunova

 I am Dr. Yulia Reunova and I have been working as a research associate in the laboratory of Dr. Russell Wyeth for the past two years, studying the neuroanatomy of the great pond snail, Lymnaea stagnalis, using Immunohistochemistry and in situ hybridization chain reaction, and combining the two to label target RNAs and proteins in the central and peripheral nervous system. My previous experience lies in the field of reproductive biology, using molecular biology methods and scanning and transmission electron microscopy. My passion is mastering new and challenging methods.

Ryan Bergman

I am an international master’s student joining the Wyeth Lab from sunny South Florida. My research involves the use of underwater videography to study juvenile lobster abundance and behavioral patterns along protected waters on Nova Scotia’s northern coastline. Earning my bachelor’s degree from the University of Florida, I studied disease ecology and physiology in a variety of marine invertebrates through research experiences. After graduating in 2023, I followed the field of crustacean biology to the University of Maine, where I first interacted with the American lobster industry and researched settlement through an internship with The Lobster Institute. I am excited to research lobsters in their early life stages here in Nova Scotia and contribute to the dynamic team at the Wyeth Lab at StFX.

Lexy Reinhardt

In the Wyeth Lab, my research focuses on the field behavior and navigation of the captivating sea slug, Hermissenda crassicornis. I earned my undergraduate degree in Marine Biology from the University of British Columbia in 2023, where I gained fieldwork skills at the Bamfield Marine Sciences Centre. Throughout my undergraduate journey, I collaborated with various organizations and labs, including Project Seahorse, iSeahorse, the O’Connor Lab, the Beaty Biodiversity Museum, the UBC Aqua Society, the UBC Women in Science Club, Women in Ocean Science, the Research Experience Program, X-Oceans Outreach, and the Sustainability Committee. As an enthusiastic diver since 2011, I have since gained my scientific diving certification, which complements my current project of capturing underwater videos of sea slugs in their natural habitats near Bamfield, British Columbia. Beyond my research, I have a deep passion for the arts, often dedicating my weekends to underwater photography and pottery.

Lauren Pictou

My name is Lauren Pictou, and I am going into my fourth-year at StFX. I am from Yarmouth, Nova Scotia and at StFX I am pursuing an honours degree in biology. Last year I began my research journey in the Wyeth lab, using Immunohistochemistry and in situ Hybridization Chain Reaction I mapped several types of neurons in the brain of Lymnaea stagnalis. After acquiring some unexpected results, I decided to focus my honours research on exploring the neurons which express choline acetyltransferase, the enzyme that synthesizes the neurotransmitter acetylcholine. I am very excited to continue to develop my hands-on skills and learn more biology!